#7595. CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

October 2026publication date
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Journal’s subject area:
Environmental Engineering;
Biomedical Engineering;
Molecular Biology;
Cell Biology;
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Abstract:
T7 Expression System is a common method of ensuring tight control and high-level induced expression. However, this system can only work in some bacterial strains in which the T7 RNA Polymerase gene resides in the chromosome. In this study, we successfully introduced a chromosomal copy of the T7 RNA Polymerase gene under control of the lacUV5 promoter into Escherichia coli BW25113.
Keywords:
5-Aminolevulinic Acid; CRISPR/cas9; E. coli; Fluorescent Protein; promoter variants; T7 Expression System

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