#7365. CRISPR/Cas12a based fluorescence-enhanced lateral flow biosensor for detection of Staphylococcus aureus

September 2026publication date
Proposal available till 13-05-2025
4 total number of authors per manuscript0 $

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Journal’s subject area:
Instrumentation;
Metals and Alloys;
Materials Chemistry;
Surfaces, Coatings and Films;
Condensed Matter Physics;
Electrical and Electronic Engineering;
Electronic, Optical and Magnetic Materials;
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Abstract:
Rapid, accurate point-of-care testing (POCT) for pathogenic bacteria detection is the key for avoiding foodborne diseases caused by pathogens or their toxins. The lateral flow biosensor (LFB) based on clustered regularly interspaced short palindromic repeats (CRISPR/Cas) has displayed remarkable potential for pathogen diagnosis. In this study, we report a novel CRISPR/Cas12a-based fluorescence enhanced LFB in conjunction with functionalized quantum dots, combined with recombinase-assisted amplification (RAA), to establish low-cost, simple, and sensitive detection of Staphylococcus aureus.
Keywords:
CRISPR/Cas12a; Lateral flow biosensor; Nucleic acid detection; Quantum dot; Staphylococcus aureus

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