#6843. Revealing the mechanisms of alkali-based magnetic nanosheets enhanced hydrogen production from dark fermentation: Comparison between mesophilic and thermophilic conditions

December 2026publication date
Proposal available till 26-05-2025
4 total number of authors per manuscript0 $

The title of the journal is available only for the authors who have already paid for
Journal’s subject area:
Renewable Energy, Sustainability and the Environment;
Bioengineering;
Environmental Engineering;
Waste Management and Disposal;
Places in the authors’ list:
place 1place 2place 3place 4
FreeFreeFreeFree
2350 $1200 $1050 $900 $
Contract №6843.1 Contract №6843.2 Contract №6843.3 Contract №6843.4
1 place - free (for sale)
2 place - free (for sale)
3 place - free (for sale)
4 place - free (for sale)

Abstract:
In the present study, a dark fermentation system inoculated with mixed culture bacteria (MCB) was developed using prepared alkali-based magnetic nanosheets (AMNSs) to facilitate biohydrogen (BioH2) production. The highest BioH2 yields of 232.8 ± 8.5 and 150.3 ± 4.8 mL/g glucose were observed at 100 (mesophilic condition) and 400 (thermophilic condition) mg/L AMNSs groups, which were 65.4% and 43.3%, respectively, above the 0 mg/L AMNSs group. The fermentation pathway revealed that AMNSs enhanced the butyrate-type metabolic pathway and the corresponding nicotinamide adenine dinucleotides (NADH and NAD+) ratio, and hydrogenase activity was enhanced in mesophilic fermentation. The interaction of AMNSs and MCB suggested that AMNSs could assist in electron transfer and that the released metal elements might be responsible for elevated hydrogenase activity. AMNSs also promoted the evolution of the dominant microbial community and altered the content of extracellular polymers, leading to increased production of BioH2.
Keywords:
Alkali-based magnetic nanosheets; Biohydrogen production; Dark fermentation; Dominant microbial communities; Hydrogenase activity

Contacts :
0