#5800. Hybrid-model-based design of fill-freeze-thaw processes for human induced pluripotent stem cells considering productivity and quality

July 2026publication date
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Journal’s subject area:
Chemical Engineering (all);
Computer Science Applications;
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Abstract:
This work presents a hybrid-model-based design of a fill-freeze-thaw processes for human induced pluripotent stem (hiPS) cells. For freezing, we applied our previously presented single-cell model, which describes heat transfer, dehydration, and crystallization during freezing. New models have been developed for filling and thawing. The accumulation of intracellular reactive oxygen species during filling and thawing was defined as the quality indicator and was described as a black-box model. Experimental results provided the necessary parameter values. The time duration for filling and thawing was defined as the productivity indicator based on physical models.
Keywords:
Cryopreservation; Grey-box model; Manufacturing; Numerical simulation; Process design; Regenerative medicine

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