#11565. Evidence of co-amplification of microbial DNA affecting interpretation at the D16S539 locus in the PowerPlex® ESI 17 Fast kit?: A case report

August 2026publication date
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Law;
Pathology and Forensic Medicine;
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More details about the manuscript: Science Citation Index Expanded or/and Social Sciences Citation Index
Abstract:
The PowerPlex® ESI 17 Fast kit (Promega Corporation) and the AmpFlSTR NGM SElect™ kit (ThermoFisher Scientific) are both used widely in the United Kingdom to generate DNA profiles for both evidential and intelligence purposes. During routine casework, we have encountered a number of cases in which otherwise normal DNA profiles prepared using the PowerPlex® ESI 17 Fast kit showed an anomalous band pattern at the D16S539 locus that was not easily explained either as a PCR/CE artifact or as a genetic anomaly. A common feature of these cases was that the exhibits sampled had once been damp and were often in a soiled condition. Some exhibits showed visual evidence of fungal/microbial growth (hereafter ‘mould’). We report here on one such case. The exhibit in this case was an abandoned item of clothing where the investigators sought to identify a potential wearer.
Keywords:
DNA profiles; The PowerPlex® ESI; AmpFlSTR NGM SElect™; intelligence purposes

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